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KMID : 0356219990300010012
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Journal of the Korean Military Medical Association
1999 Volume.30 No. 1 p.12 ~ p.20
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Seroepidemiologic Study of Anti-Pv200 Antibody Positive Soldiers in Vivax Malaria Endemic Areas
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노천섭/Rho, Cheon Seop
박채규/최춍준/황보영/정희생/김영자/김승육/Park, Chae Gyu/Chwae, Yong Joon/Hwangbo, Young/Jung, Hee Saeng/Kim, Young Ja/Kim, Seung Yuck
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Abstract
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Anti-Pv200 antibody levels were tested in the samples from endemic areas of vivax malaria recently outbreaked in Korea, using indirect ELISA method and asymptomatic carriers of Plasmodium vivax in their blood were detected using nested PCR. Anti-Pv200 antibody titers in 20 vivax malaria patients were markedly higher than those of normal control group(O.D. value 0.08±0.16 of IgG type and O.D. value 0.04±0.04 of IgM type) to O.D. value 1.85±0.29 of IgG type and O.D. value 1.33±1.33 of IgM type. Through the comparison of antibody titers between patients and normal control group, O.D. value 0.5 of IgG type and O.D. value 0.2 of IgM type were defined as cut-off values and anti-Pv-200 levels were measured in the various groups. Antibody titers of 8 soldiers with past malaria history were sustained to above cut-off values until the period ranged from 4 month to 6 month after malaria attack and in vivax malaria attacked 40 soldiers within 3 months after our sampling showed 11 positive samples( 27.5 %) compared to 5 positive samples(12.5%) of randomly selected 40 soldiers who were not attacked by vivax malaria within 3 months after our sampling. This result suggests that possibly antibody responses against merozoite surface protein-1in, the liver: stage of vivax malaria infection can be occured. Antiboty positive ratio of 1,700 soldiers serving at high risk areas of vivax malaria was 15%. Among 800 blood samples from asymptomatic sodiers serving at high risk areas tested about the presence of Plasmodium vivax by nested PCR amplification, 4 positive samples(0.5%) were detected. Our results show that anti-Pv-200 antibody test by indirect ELISA method can be the useful diagnostic tool detecting vivax malaria in Korea, used efficiently in seroepidemiologic study of large number of people, and asymptomatic Plasmodium vivax carriers in their blood can be existed as the results of nested PCR amplification.
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